LiquidHALLMARK® ctDNA and ctRNA Targets List

LiquidHALLMARK® is a sensitive next-generation sequencing assay targeting both circulating DNA and RNA to profile a patient’s unique cancer. LiquidHALLMARK profiles ctDNA mutations in 80 genes, fusions in 10 genes, and somatic variants. As an add-on option, LiquidHALLMARK analyses 27 ctRNA targets for actionable and emerging fusions.

Download Gene List here
 

Genes*
ABL1 CCND2 # FBXW7 # IDH1 MED12 PDGFRA # RIT1
AKT1 CDH1 FGFR1 IDH2 MET # PIK3CA # ROS1
ALK # CDK6 # FGFR2 JAK1 MLH1 PIK3R1 SF3B1
APC CDKN2A # FGFR3 JAK2 MTOR PPP2R1A SMAD4 ^
AR CREBBP FLT3 JAK3 MYC # PTEN # SMO
ARAF CTNNB1 GATA3 KEAP1 1 NF1 PTPN11 SPOP
ATM EGFR †# GNA11 KIT # NFE2L2 RAF1 STK11
BRAF ERBB2 # (HER2) GNAQ KRAS # NOTCH1 RB1 TERT Promoter
BRCA1 #1 ERCC2 GNAS MAP2K1 (MEK1) NRAS # RET TP53 ^
BRCA2 #2 ESR1 HNF1A MAP2K2 (MEK2) NTRK1 RHEB U2AF1
CCND1 # EZH2 HRAS MAPK1 (ERK2) NTRK3 RHOA VHL
Fusions
ALK AR R (AR-V7 SV) BRAF R CTNNB1-PLAG1 R EGFR R ERBB4 R ERG R
ESR1 R ETV1 R ETV4 R ETV5 R FGFR1 R FGFR2 FGFR3
FLI1 R MET (+Ex14 Skipping) MYB-NFIB R NRG1 R NTRK1/2/3 PAX3-FOXO1 R PAX8-PPARG R
PD-L1 D (CD274) RET ROS1 SSX1 R SSX2 R TMPRSS2 D
MSI
BAT25 BAT26 NR21 NR24 NR27 MONO27
Viruses
Epstein-Barr Virus (EBV) Hepatitis B Virus (HBV) Human Herpesvirus (HHV-8)

 
 
* Targeted regions selected to maximize detection of known hotspot mutations. # Includes detection of gene copy number changes. Includes sequencing of EGFR kinase and extracellular domain mutations. ^ Full coverage. 1 >99% coverage. 2 >98.4% coverage of coding exons. R Fusions analysed by ctRNA only. D Fusions analysed by ctDNA only. SV Splice Variant
Fusions highlighted in orange are analyzed using both ctDNA and ctRNA. ctRNA results are currently investigational – results provided for informational, non-diagnostic purposes only.