LiquidHALLMARK® ctDNA and ctRNA Targets List

LiquidHALLMARK® is a sensitive next-generation sequencing assay targeting both circulating DNA and RNA to profile a patient’s unique cancer. LiquidHALLMARK profiles ctDNA mutations in 80 genes, fusions in 10 genes, and somatic variants. As an add-on option, LiquidHALLMARK analyses 36 ctRNA targets for actionable and emerging fusions.

Download Gene List here

 

Genes*

ABL1 CCND2 # FBXW7 # IDH1 MED12 PDGFRA # RIT1
AKT1 CDH1 FGFR1 IDH2 MET # PIK3CA # ROS1
ALK # CDK6 # FGFR2 JAK1 MLH1 PIK3R1 SF3B1
APC CDKN2A # FGFR3 JAK2 MTOR PPP2R1A SMAD4 ^
AR CREBBP FLT3 JAK3 MYC # PTEN # SMO
ARAF CTNNB1 GATA3 KEAP1 1 NF1 PTPN11 SPOP
ATM EGFR †# GNA11 KIT # NFE2L2 RAF1 STK11
BRAF ERBB2 # (HER2) GNAQ KRAS # NOTCH1 RB1 TERT Promoter
BRCA1 #1 ERCC2 GNAS MAP2K1 (MEK1)    NRAS # RET TP53 ^
BRCA2 #2 ESR1 HNF1A MAP2K2 (MEK2) NTRK1 RHEB U2AF1
CCND1 # EZH2 HRAS MAPK1 (ERK2) NTRK3 RHOA VHL
 

Fusions (ctDNA)

ALK FGFR2 FGFR3 NTRK1/2/3 PD-L1 (CD274)      RET ROS1
TMPRSS2
 

Fusions (ctRNA)

ALK AR  (V3,4,7 & 9 SV)     AXL-MBIP BRAF CLIP1-LTK    CTNNB1-PLAG1    DNAJB1-PRKACA
EGFR ERBB4 ERG ESR1 ETV1/4/5 FGFR1 FGFR2
FGFR3 FLI1 MET (+Ex14 Skipping)      MYB-NFIB NRG1 NTRK1/2/3 PAX3-FOXO1
PAX8PPARG      RET ROS1 RSPO2 RSPO3 SLC45A3 SSX1
SSX2 TFE3 THADA TMPRSS2
 

Microsatellite Instability (MSI)

BAT25 BAT26 NR21 NR24 NR27 MONO27

 

* Targeted regions selected to maximize detection of known hotspot mutations. # Includes detection of gene copy number changes. Includes sequencing of EGFR kinase and extracellular domain mutations. ^ Full coverage. 1 >99% coverage. 2 >98.4% coverage of coding exons. SV Splice Variant
Fusions highlighted in orange are analyzed using both ctDNA and ctRNA. ctRNA results are currently investigational – results provided for informational, non-diagnostic purposes only.